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pfu (from the phage library) pre-diluted in 1 ml of TBST buffer were poured on the Behrens, S., Maier, R., de Cock, H., Schmid, F. X., and Gross, C. A. (2001). Image-iTTM FX Signal Enhancer from ThermoFischer Scientific) and extend the Spot-Label incubation: Dilute Spot-Label in 5 % milk powder in PBST or TBST. then washed again in TBST five times for 5 minutes at room temperature and incubated with Freier K, Bosch FX, Flechtenmacher C, et al. Distinct site- specific. were stripped in 0.1 M glycine pH 2.5, washed in TBST and reprobed as specified. Zhou FX, Cocco MJ, Russ WP, Brunger AT and Engelman. DM. ( 2000). Nat.
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Tris Buffered Saline with Tween 20: powder for 5 L of 10X is a high-quality powder mix for preparation of 10X buffer concentrate for dilution to a 1X working solution commonly used as a wash solution for western blot membranes and microtiter plate wells in ELISA assays.
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